diff --git a/nixos/doc/manual/release-notes/rl-2405.section.md b/nixos/doc/manual/release-notes/rl-2405.section.md
index 93a9da175b1e..e545bda2fba3 100644
--- a/nixos/doc/manual/release-notes/rl-2405.section.md
+++ b/nixos/doc/manual/release-notes/rl-2405.section.md
@@ -282,6 +282,8 @@ The pre-existing [services.ankisyncd](#opt-services.ankisyncd.enable) has been m
   "mysecret"` becomes `services.aria2.rpcSecretFile = "/path/to/secret_file"`
   where the file `secret_file` contains the string `mysecret`.
 
+- The `system.forbiddenDependenciesRegex` option has been renamed to `system.forbiddenDependenciesRegexes` and now has the type of `listOf string` instead of `string` to accept multiple regexes.
+
 - `openssh`, `openssh_hpn` and `openssh_gssapi` are now compiled without support for the DSA signature algorithm as it is being deprecated upstream. Users still relying on DSA keys should consider upgrading
   to another signature algorithm. However, for the time being it is possible to restore DSA key support using `override` to set `dsaKeysSupport = true`.
 
diff --git a/nixos/modules/profiles/perlless.nix b/nixos/modules/profiles/perlless.nix
index 90abd14f077e..010e4f8f2a28 100644
--- a/nixos/modules/profiles/perlless.nix
+++ b/nixos/modules/profiles/perlless.nix
@@ -26,6 +26,6 @@
 
   # Check that the system does not contain a Nix store path that contains the
   # string "perl".
-  system.forbiddenDependenciesRegex = "perl";
+  system.forbiddenDependenciesRegexes = ["perl"];
 
 }
diff --git a/nixos/modules/system/activation/test.nix b/nixos/modules/system/activation/test.nix
index 8cf000451c6e..fd251d528957 100644
--- a/nixos/modules/system/activation/test.nix
+++ b/nixos/modules/system/activation/test.nix
@@ -5,7 +5,7 @@
 }:
 let
   node-forbiddenDependencies-fail = nixos ({ ... }: {
-    system.forbiddenDependenciesRegex = "-dev$";
+    system.forbiddenDependenciesRegexes = ["-dev$"];
     environment.etc."dev-dependency" = {
       text = "${expect.dev}";
     };
@@ -14,7 +14,7 @@ let
     boot.loader.grub.enable = false;
   });
   node-forbiddenDependencies-succeed = nixos ({ ... }: {
-    system.forbiddenDependenciesRegex = "-dev$";
+    system.forbiddenDependenciesRegexes = ["-dev$"];
     system.extraDependencies = [ expect.dev ];
     documentation.enable = false;
     fileSystems."/".device = "ignore-root-device";
diff --git a/nixos/modules/system/activation/top-level.nix b/nixos/modules/system/activation/top-level.nix
index 4cf3012646fa..ed0ece19f2fa 100644
--- a/nixos/modules/system/activation/top-level.nix
+++ b/nixos/modules/system/activation/top-level.nix
@@ -86,6 +86,7 @@ in
     ../build.nix
     (mkRemovedOptionModule [ "nesting" "clone" ] "Use `specialisation.«name» = { inheritParentConfig = true; configuration = { ... }; }` instead.")
     (mkRemovedOptionModule [ "nesting" "children" ] "Use `specialisation.«name».configuration = { ... }` instead.")
+    (mkRenamedOptionModule [ "system" "forbiddenDependenciesRegex" ] [ "system" "forbiddenDependenciesRegexes" ])
   ];
 
   options = {
@@ -160,12 +161,12 @@ in
       '';
     };
 
-    system.forbiddenDependenciesRegex = mkOption {
-      default = "";
-      example = "-dev$";
-      type = types.str;
+    system.forbiddenDependenciesRegexes = mkOption {
+      default = [];
+      example = ["-dev$"];
+      type = types.listOf types.str;
       description = ''
-        A POSIX Extended Regular Expression that matches store paths that
+        POSIX Extended Regular Expressions that match store paths that
         should not appear in the system closure, with the exception of {option}`system.extraDependencies`, which is not checked.
       '';
     };
@@ -289,15 +290,14 @@ in
             "$out/configuration.nix"
         '' +
       optionalString
-        (config.system.forbiddenDependenciesRegex != "")
-        ''
-          if [[ $forbiddenDependenciesRegex != "" && -n $closureInfo ]]; then
-            if forbiddenPaths="$(grep -E -- "$forbiddenDependenciesRegex" $closureInfo/store-paths)"; then
+        (config.system.forbiddenDependenciesRegexes != []) (lib.concatStringsSep "\n" (map (regex: ''
+          if [[ ${regex} != "" && -n $closureInfo ]]; then
+            if forbiddenPaths="$(grep -E -- "${regex}" $closureInfo/store-paths)"; then
               echo -e "System closure $out contains the following disallowed paths:\n$forbiddenPaths"
               exit 1
             fi
           fi
-        '';
+        '') config.system.forbiddenDependenciesRegexes));
 
     system.systemBuilderArgs = {
 
@@ -319,8 +319,7 @@ in
       # option, as opposed to `system.extraDependencies`.
       passedChecks = concatStringsSep " " config.system.checks;
     }
-    // lib.optionalAttrs (config.system.forbiddenDependenciesRegex != "") {
-      inherit (config.system) forbiddenDependenciesRegex;
+    // lib.optionalAttrs (config.system.forbiddenDependenciesRegexes != []) {
       closureInfo = pkgs.closureInfo { rootPaths = [
         # override to avoid  infinite recursion (and to allow using extraDependencies to add forbidden dependencies)
         (config.system.build.toplevel.overrideAttrs (_: { extraDependencies = []; closureInfo = null; }))